羅能文 副教授

Lo, Neng-Wen

Email:nlo@thu.edu.tw

羅能文(第六家族)Lo, Neng-Wen
職稱
Title
副教授
Associate Professor
學生訪談時間
Students time
星期二 10:00~12:00 ; 星期三 16:00~17:00
Tues. 10:00~12:00 Wed. 16:00~17:00
聯絡方式
Contact
Tel: 886-04-23590121轉37115
Fax: 886-04-23590385 
E-mail: nlo@thu.edu.tw
學經歷
Background
東海大學物理學系學士
美國紐約州立大學生物化學系博士
美國約翰霍普金斯醫學院博士後研究員
B.S., Dept. of Physics, Tunghai University
Ph.D., State University of New York at Buffalo, USA
Fellow, Dept. of Tumor Biology, Johns Hopkins School of Medicine, USA
研究方向
Research
利用生物資訊工具對基因庫作系統性搜尋的結果,可以找到須費時數年才能找到的未知基因並獲致生化相關重要的發現,乃生物醫學的研究趨勢之一。在約翰霍普金斯醫學院擔任博士後研究員的研究著作(Lo, N.W., Shaper, J.H., Pevsner, J. & Shaper, N.L. (1998). The expanding Beta4-galactosyltransferase gene family: messages from the databanks. Glycobiology, 8(5):517-26. (SCI))中,便詳盡的描述可如何利用生物資訊獲得的資料結合分子生物學的技術迅速找到新的且完整的Beta4-半乳糖轉移酵素基因(Beta4-GT)密碼序列,而後在實驗室裡利用分生技術,以昆蟲細胞進行蛋白質的表現、並建構出基因家族樹探討它們與其他Beta4-GT在演化上的關係,而後並對這些成員對擔綱乳糖合成的角色作深入探討。而這篇研究報告所獲致的訓練將本人的研究興趣帶至三個主要的方向:

1、以生物資訊探討啟動子的特徵:
主要成果:利用基因演算法,找到了數千個重要的啟動子組成序列,利用這些共通序列作為啟動子之特徵開發設計出一種電腦分析軟體,用以預測未知啟動區(作品「ProDictor;啟動子預測分析軟體」公佈於http://140.128.107.247/index.html,正式發表於美國拉斯維加斯2003年『數學與工程在生物醫學之應用』研討會)。本研究成果已在兩個國際研討會發表,整體成果正在整理成期刊論文發表中。若能以電腦分析的方式定義所有基因啟動區所帶有的核酸序列模式,便可協助找到所有的基因的起點。而且啟動區對基因轉殖動物欲於特定組織專一表現特定基因的任務,具有不可或缺的重要地位。

2、X-精子(或稱X精蟲)與Y-精子(Y精蟲)的分離技術開發:
欲達到控制子代性別的最佳方式是將決定性別的X-精子與Y-精子事先加以分離,再令其與卵子進行受精作用,而後產生大批飼主偏愛之性別的下一代。利用X-精子與Y-精子間具有的些微理化特性上的差異,諸如帶電荷數與游動速率的不同等等,數種分離精子的技術,自1970年代相繼被推出,包括discontinuous albumin gradients、sephadex columns、discontinuous percoll gradients、swim-up procedure與flow cytometry等。分離精子的方法雖多,然皆未臻完善,發展至今接受性尚不普及,仍舊有許多留待改善的空間。有鑑於此,利用生物技術,以基因轉殖的方式,將外源性蛋白質標記物(protein marker)放入X-精子或Y-精子內,予以大量的表現,或許可達到有效分離大型動物X-與Y-精子的目的。代表作是尋獲世界上第一個可以鑑定X-精子的標定蛋白質(marker protein)。(Yueh-Chiao Yeh, Vie-Cheng Yang, Shing-Chung Huang and Neng-Wen Lo.(2005) Stage-dependent expression of extra-embryonic tissue-spermatogenesis-homeobox gene1(ESX1) protein, a candidate marker for X chromosome-bearing sperm. Reproduction, Fertility and Development, 17, 447-455. )

3、乳糖生成與分解的相關研究:
主要成果:乳糖的生成需要有Beta4-半乳糖轉移酵素基因(Beta4- galactosyltransferase ,GT),利用生物資訊獲得的資料結合分子生物學的技術,本人迅速找到新的且完整的Beta4-半乳糖轉移酵素基因(Beta4-GT)密碼序列,成果已發表於Glycobiology(SCI)的專業期刊。而乳糖的分解則需要另一種酵素-半乳糖苷酶(Beta-galactosidase)的作用。本實驗室已成功地在生乳中篩選分離出具有高度活性的Beta4-半乳糖苷酶(Beta-galactosidase)的低溫菌,目前也已經從菌內分離出其基因,而基因序列與NCBI資料庫比對的結果發現,該序列從未被發表過,因此我們發現的基因先前皆未被發現過。而依DNA序列轉譯出之蛋白質序列與資料庫內的已知半乳糖苷酶蛋白質序列作比對,發現其序列相同度約為百分之六十六,顯示我們發現的基因確是屬於半乳糖酵素基因家族,目前已將蛋白質作表現與作更進一步對其分解乳糖能力作探討,結果發現其在低溫下具有極非常好的活性。
授課名稱
Courses
生物科技概論 (0-2) 
Introduction to Biotechnology (0-2) 
生物化學(3-2)
Biochemistry(3-2)
基礎分子生物學 (2-0) 
Introdution to Molecular Biology (2-0) 
生殖細胞生物學 (2-2) 
Germ Cell Biology (2-2) 
生物技術實驗 (1-1) 
Biotechniques (1-1) 
生物分子檢測 (0-2) 
Assays on Biomolecules (0-2) 
生物資訊電腦搜尋(0-2)
Computer Search of Biological Information (0-2)
  1. 以生物資訊探討啟動子的特徵
  2. X-精子(或稱X精蟲)與Y-精子(Y精蟲)的分離技術開發
  3. 乳糖生成與分解的相關研究
  1. Lin, N.S., Lin, B.Y. Lo, N.W., Hu, C.C., Chow, T.Y. & Hsu, Y.H. 1994. Nucleotide sequence of the genomic RNA of bamboo mosaic potexvirus. Journal of General Virology 75 ( Pt 9),2513-8. (SCI)
  2. Lo, N.W. & Lau, J.T. 1996. Transcription of the β-galactoside α 2,6-sialyltransferase gene in B lymphocytes is directed by a separate and distinct promoter. Glycobiology 6(3):271-9. (SCI)
  3. Lo, N.W. & Lau, J.T. 1996. Novel heterogeneity exists in the 5′-untranslated region of the -galactoside α 2,6-sialytransferase mRNAs in the human B-lymphoblastoid cell line, Louckes. Biochemical & Biophysical Research Communications 228(2):380-5. (SCI)
  4. Shaper, N.L., Charron, M., Lo, N.W. & Shaper, J.H. 1998. β4-Galactosyltransferase and lactose biosynthesis: Recruitment of a housekeeping gene from the nonmammalian vertebrate gene pool for a mammary gland specific function. J. Mammary Gland Biology and Neoplasia 3(3):315-24.(SCI)
  5. Lo, N.W., Shaper, J.H., Pevsner, J. & Shaper, N.L. 1998. The expanding β4-galactosyltransferase gene family: messages from the databanks. Glycobiology 8(5):517-26. (SCI)
  6. Lo, N.W. & Lau, J.T. 1999. Transcription of the β-galactoside 2,6-sialyltransferase gene (SIAT1) in B lymphocytes: Cell type-specific expression correlates with presence of the divergent 5’-untranslated sequence. Glycobiology 9(9): 907-914. (SCI)
  7. Lo, N.W., Dennis, J. W. & Lau., J. T. Y. 1999. Overexpression of the alpha2,6-sialyltransferase, ST6Gal I, in a low metastatic variant of a murine lymphoblastoid cell line is associated with appearance of a unique ST6Gal I mRNA. Biochemical &Biophysical Research Communications 264 (3):619-621. (SCI)
  8. Lo, N. W., Changchien, S. W., Chang, Y. F. and Lu, T. C. 2002. Human promoter prediction based on sorted consensus sequence patterns by genetic algorithms. Proceedings of the International Congress on Biological and Medical Engineering. D3I-1540: pp 111-112.
  9. 朱俐潔、蘇郁欣、施宗雄、閻立平、羅能文。2003。生乳中產 β 1,4-半乳糖分解酵素低溫菌之篩選。東海學報 44: 59-65。
  10. Lo, N. W., Changchien, S. W., Chang, Y. F. and Lu, T. C. 2003. Improved promoter prediction by two-phase neural network. Proceedings of the International Conference on Mathematics and Engineering Techniques in Medicine and Biological Science pp 42-46{ISBN = 1-932415-04-1}
  11. Chang, H. T., Lo, N. W., Lu, W. C. and Kuo, C. J. 2003. Visualization and comparison of DNA sequences by use of three-dimensional trajectories. Proceedings of the Conferences in Research and Practice in Information Technology pp 81-85. {ISBN ~ ISSN:1445-1336 , 0-909-92597-6}
  12. Chang, H.T., Xiao, S.W., Lin, C.C. & Lo, N.W. 2004. “Feature Extraction based on Three-Dimensional Trajectory (TDT) of DNA Sequences,” Workshop on Consumer Electronics and Signal Processing (WCEsp2004), pp. 459~463, 2004/11
  13. Yeh, Y. C., Yang V.C., Huang, S. C. and Lo, N. W. 2005. Stage-dependent expression of ESX1 protein: a candidate marker for X-chromosome-bearing sperm. Reproduction, Fertility and Development 17(4): 447-455. (SCI)
  14. 蘇郁欣、羅能文。2005。耐低溫Serratia屬D04菌株β1,4-半乳糖??之定性分析與基因選殖。中國畜牧學會會誌34(3):189-198。
  15. Leu, F.Y., Lo, N.W. and Yang, L.N. 2005. Predicting Vertebrate Promoters with Homogeneous Cluster Computing, IEEE SITIS, pp. 143-148.
  16. Lo, N.W., Chang, H.T., Xiao, S.W., Li, C.H. & Kuo, C.J. 2007. Global visualization and comparison of DNA sequences by use of three-dimensional trajectories. J. of Info. Sci. and Eng. 23 :1723-1736. (SCI)
  17. Intawicha, P., Ou, Y.W., Lo, N.W., Zhang, S.C., Chen, Y.Z., Lin, T.A., Su, H.L., Guu, H.F., Chen, M.J., Lee, K.H., Chiou, W.T. and Ju, J.C. 2009. Characterization of embryonic stem cell lines derived from New Zealand White rabbit embryos. Cloning and Stem Cells 11(1):27-38. (SCI)
  18. Nguyen, N. T., Lin, P.C., Siriboon, C., Lo, N. W. and Ju, J. C. 2010. Sonic Hedgehog improves in vitro development of porcine parthenotes and handmade cloned embryos. Theriogenology 74(4):1149-1160. (SCI)
  19. 謝亞振、殷永山、羅能文、李坤雄、朱志成。2010。綜合評論:源自孤雌胚胚幹細胞之特性與應用。台灣農學會報11(6):580-601。
  20. Leu, F.Y., Yang, L.N., Lo, N.W. and Lin, I. L. 2010. Predicting vertebrate promoters using heterogeneous clusters. Int. J. of Ad Hoc and Ubiquitous Computing 6(4): 216-234. (SCI)
  21. Hsieh, Y.C., Intawicha P., Lee, K.H., Chiu, Y.T., Lo, N.W. and Ju, J.C. 2011. LIF and FGF Cooperatively Support Stemness of Rabbit Embryonic Stem Cells Derived from Parthenogenetically Activated Embryos. Cell Reprogram 13(3):241-55.(SCI)
  22. Nguyen, N.T., Lo, N.W., Chuang, S. P., Jian, Y.L. and Ju, J. C. 2011. Sonic Hedgehog supplemented oocyte and embryo culture media enhances development of in vitro fertilized porcine embryos. Reproduction 142(1):87-97.(SCI)
  23. Chawalit, S., Nguyen, N.T., Tseng, J.K., Lo, N.W., Tu, C.F., and Ju, J.C. 2012. Trichostatin A and ascorbic acid assist in the development of porcine handmade cloned embryos via different physiologic pathways. Reprod Sci. 19(9):976-86.
  24. Chang, H.T., Kuo, C. J., Lo, N.W., Lv, W.Z. 2012. DNA Sequence Representation and Comparison Based on Quaternion Number System. Int. J. Adv. Comput. Sci. Appl. 3(11): 39-46.
  25. Kere, M., Siriboon, C., Lo, N.W., Nguyen, N.T., and Ju, J.C. 2013. Ascorbic Acid Improves the Developmental Competence of Porcine Oocytes after Parthenogenetic Activation and Somatic Cell Nuclear Transplantation. J. Reprod. Dev. 59(1):78-84.
  26. Intawicha P, Wang SH, Hsieh YC, Lo NW, Lee KH, Huang SY, Ju JC. 2013. Proteomic profiling of rabbit embryonic stem cells derived from parthenotes and fertilized embryos. PLoS One. 8(7):e67772.
  27. Kere M, Siriboon C, Liao JW, Lo NW, Chiang HI, Fan YK, Kastelic JP, Ju JC. 2014. Vascular endothelial growth factor A improves quality of matured porcine oocytes and developing parthenotes. Domest Anim Endocrinol. 49C:60-69.
  28. Siriboon C, Tu CF, Kere M, Liu MS, Chang HJ, Ho LL, Tai ME, Fang WD, Lo NW, Tseng JK, Ju JC. 2014. Production of viable cloned miniature pigs by aggregation of handmade cloned embryos at the 4-cell stage. Reprod Fertil Dev. 26(3):395-406.
  29. Lo, N.W., Intawicha, P., Chiu, Y.T., Lee, K.H., Lu, H.C. , Chen, C.H., Chang,Y.H., Chen, C.D. and Ju, J.C. 2015. Leukemia Inhibitory Factor andFibroblast Growth Factor-2 Critically and Mutually Sustain Pluripotency of
    Rabbit Embryonic Stem Cells. Cell Transplantation.
  30. Intawicha, P., Siriboon, C., Chen, C.H., Chiu, Y.T., Lin, T.A., Kere, M., Lo,N.W., Lee, K.H., Chang, L.Y., Chiang, H.I.,and Ju, J.C. 2016. Derivation andcharacterization of putative embryonic stem cells from cloned rabbit embryos.Theriogenology 86(7):1799-810.
  31. Wang, D.C., Huang, J.C., Lo, N.W., Chen, L.R., Mermillod, P., Ma, W.L.,Chiang, H.I., and Ju, J.C. 2017. Sonic Hedgehog promotes in vitro oocytematuration and term development of embryos in Taiwan native goats.
    Theriogenology 103:52-58.
  32. Lumsangkul, C., Chiang, H.I., Lo, N.W., Fan, Y.K., and Ju, J.C. 2019.Developmental Toxicity of Mycotoxin Fumonisin B₁ in Animal Embryogenesis: An Overview. Toxins 13:11(2). pii: E114. doi: 10.3390/toxins11020114. Review.​(MOST 106-2313-B-039-003-MY2.)
  33. Intawicha, P., Tsai, L.K., Yen, S.Y., Lo, N.W., and Ju, J.C. 2019. Nucleus,Cytoskeleton, and Mitogen-Activated Protein Kinase p38 Dynamics during In Vitro Maturation of Porcine Oocytes. Animals 13;9(4). pii: E163. doi: 10.3390/ani9040163.(MOST 106-2313-B-039-003-MY2.)
  1. Grollman, E.F., Lo, N.W. & Lau, J.T. 1992. Regulated expression of β-galactoside 2,6 sialyltransferase in rat FRTL-5 cells. Glycobiology 2, 478
  2. 2.Lo, N.W., Vertino-Bell, A., Hu, Y.P. & Lau, J.T. 1994. 5’ heterogeneity in mRNAs expressed from the β-galactoside 2,6-sialyltransferase gene, Glycobiology 4, 739.
  3. Lo, N.W. & Lau, J.T. 1995. Transcriptional regulation of β-galactoside 2,6-sialyltransferase in B lymphocytes. Glycoconjugate J. 12, 470.
  4. Lo, N.W. & Lau, J.T. 1996. Transcriptional regulation of β-galactoside 2,6-sialyltransferase in B lymphocytes. Glycobiology 6, 721.
  5. Lo, N.W., Shaper, J.H., Pevsner, J.& Shaper, N.L. 1998. The mouse β4-galactosyltransferase gene family. FASEB 12, A1348.
  6. Lo, N.W., Shaper, J.H. & Shaper, N.L. 1999. Differential expression of the murine β1,4-galactosyltransferase gene family members in male germ cells during spermatogenesis. c 16, S73.
  7. Lo, N.W., Shaper, J.H., Pevsner, J.& Shaper, N.L. (1999). The mouse β4-galactosyltransferase gene family. J. Chin. Soc. Anim. Sci. 28 (Suppl.): 79.
  8. Lo, N.W., Shaper, J.H. & Shaper, N.L. 2000. Differential expression of the murine β1,4-galactosyltransferase gene family members in murine male germ cells. J. Chin. Soc. Anim. Sci. 29 (Suppl.): 148.
  9. Huang, S.C., Hu, C.C. & Lo, N.W. 2000. Generation of polyclonal antibodies against the mouse homeobox protein Esx1. J. Chin. Soc. Anim. Sci. 29 (Suppl.): 149.
  10. Ku, M.C. & Lo, N.W. 2000. Tissue-specific expression of the Esx1 gene correlates with its heterogeneous mRNA isoforms. J. Chin. Soc. Anim. Sci. 29 (Suppl.): 150.
  11. Chu, L.C., Shih, T.H. & Lo, N.W. 2001. Characterization of the β1,4-galactosidase activity in psychrotrophs. J. Chin. Soc. Anim. Sci. 30 (4): 113.
  12. Ku, M.C. & Lo, N.W. 2001. Effects of stress on Esx1 gene expression in embryogenesis. J. Chin. Soc. Anim. Sci. 30 (4): 194.
  13. Yeh, Y. C., Huang, S. C. and Lo, N. W. 2002. Stage-dependent expression of the esx1 in mouse testis. Proceedings of the 4th Asian-Pacific Organization for Cell Biology Congress. POS9-5: pp257.
  14. Wang, P.Y. & Lo, N.W. 2002. Cloning of the genes that differentiate primary spermatocytes from spermatogonia. J. Chin. Soc. Anim. Sci. 31(4):70.
  15. Yeh, Y.C., Huang, S. C. & Lo, N.W. 2002. The apecific expressionof the Esx1 in the testis and spermatozoa. J. Chin. Soc. Anim. Sci. 31(4):198.
  16. Lo, N.W., Changchien, S.W., Chang, Y.F. 2003. Messages from the sequence comparison between the transcription factor binding sites and extracted promoter consensus sequences. Proceedings of the2003 Bioinformatics in Taiwan, Symposium and Workshop. pp110.
  17. Lo, N. W., Chang, Y. F. and Changchien, S. W. 2004. Sequence comparison between the transcription factor binding sites and extracted promoter consensus sequences. Proceedings of the Yeast Genetics and Molecular Biology Meeting 2004. pp.227.
  18. Chang, H.T., Xiao, S.W. & Lo, N.W. 2005 Feature extraction and comparison of TDTs: an efficient sequence retrieval algorithm for genomic databases. Proceedings of the Third Asia-Pacific Bioinformatics Conference & Singapore Bioinformatics Week. pp75.
  19. Yeh, Y.C., Yang, V.C. and Lo, N.W. 2005. In vitro analyses of the putative promoter region of Esx1 gene in testis and placenta. Proceedings of the 38th Annual Meeting of the Society for the Study of Reproduction 164, pp118-119.
  20. Lo, N.W. , Cheng, R.S., Yeh, Y.C. and Yang, V.C. 2007 Characterization of the regulatory elements of the spermatogenesis-specific Esx1 gene. BIOLOGY OF REPRODUCTION 77, 117-a–117.
  21. Wang, M.C., Lo, N.W.& Chang, H.T. 2008. Monitored and inspected mouse mating by video surveillance. Journal of The Chinese Society of Animal Science , vol. 37(Supplement), pp. 113, Taipei, 2008/12/7.
  22. Hsieh, Y.C., Intawicha, P., Lo, N.W., Lee, K.H. and Ju, J.C. 2009. Establishment and characterization of rabbit embryonic stem cell lines derived from parthenogenetically activated embryos. “The International Symposium of Stem Cells and Bioengineering” on the 16th and 17th of May of 2009 at the Convention Center of Medical College of National Cheng Kung University. P2-17.
  23. Lo, N. W., Chang, H.T. & Wang, M.C. 2009. Automatic Detection of Mouse Mating Behavior by Video Surveillance,” APSIPA Annual Summit and Conference (APSIPA ASC 2009), Sapporo, Japan, October 4-7, TP-P2-5: 0149.
  24. 24.Hsieh,Y.C., Intawicha, P., Lo, N.W. and Ju, J. C. 2009. Establishment and characterization of rabbit embryonic stem cell lines derived from parthenogenetically activated embryos.Annual meeting of Taiwan Society for Stem Cell Research, TSSCR 2009.(Suppl.):116.
  25. 25.Intawicha, P., Hsieh, Y.C., Lo, N.W., Lee, K.H. and Ju, J. C. 2010. Synergistic integration of LIF and FGF pathway supports self-renewal of Rabbit embryonic stem cells. 3rd Conference of the Asia Pacific Initiative on Reproduction, ASPIRE 2010.
  26. 26.Ju, J.C., Intawicha, P., Wang, S.H., Hsieh, Y.J., Lo, N.W. and Huang, S.Y. 2010. Proteomic Analysis of Rabbit Embryonic Stem Cells Derived from Fertilized and Parthenogenetically Activated Embryos. Biology of Reproduction (Suppl):199-200.
  27. Lo, N.W., Intawicha P., Hsieh, Y. C., Lee, K.H., Chen, L.R. and Ju, J.C. 2010. Cloning rabbit embryonic stem cells. 14th Animal Science Congress of the Asian-Australasian Association of Animal Production Societies (14th AAAP Animal Science Congress). (Suppl. 1) : 249.
  28. Intawicha, P., Pan, Y.H.,Chen, C.D.,Hsieh, Y.C. Tseng, J.K., Lo, N. W.,Lee, K. H. and Ju, J. C. 2010. A pilot study of intracellular calcium profiling and cell cycle synchronization in rabbit embryonic stem cells. The 8th Annual Symposium of Bioinformatics and Systems Biology in Taiwan, BIT 2010. p.75.
  29. N.W. Lo* (羅能文), C.A. Cheng, C.Y. Huang (黃啟裕), S.Y. Wang, and Y.H. Chen (陳盈豪).108.Effect of methanotropic bacteria on methane production by Taiwan male black-feathered native chickens The 2nd International Conference on Native Chicken.(ICONC 2019)泰國
  30. Chen YH, Chueh HC, Haung CY (黃啟裕)*, Lo NW (羅能文).107.Denitrification of the aerobic methanotroph Methylomonas strain THUA isolated from rumen.11th Asian Symposium on Microbial Ecology. (ASME-2019)101-101台灣
  31. J.C. Ju*, C.R. Yang, P. Intawicha, N.W. Lo (羅能文) and H.I.Chiang.104.Embryoid bodies as a model system to study effects of heat stress on post-implantation development of mammalian embryos.1st International Conference on Tropical Animal Science and Production.(TASP 2016)90泰國
  32. 闕浩丞、韓昕彤、陳怡璇、黃啓裕、江信毅、魏天恩、羅能文*。106。瘤胃嗜甲烷菌體外培養條件與 碳源降解能力最佳化研究。中國畜牧年會。46。115-台灣
  1. Shaper, N.L., Charron, M., Lo, N.W., & Shaper, J.H. (1998). β4-Galactosyltransferase and lactose biosynthesis: Recruitment of a housekeeping gene from the nonmammalian vertebrate gene pool for a mammary gland specific function. J. Mammary Gland Biology and Neoplasia, 3(3):315-24.(SCI) (review)
  2. Shaper, N.L., Charron, M., Lo, N.-W., Scocca, J.R., & Shaper, J.H. (2000). The Galactosyltransferases. In “Carbohydrates in Chemistry and Biology: A Comprehensive Handbook”, Part II (vol. III), Ernst, B., Hart, G., and Sinay, P., eds. Wiley/VCH press, New York. Chapter 10, 175-196. (book chapter)
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