羅能文 副教授暨系主任

Lo, Neng-Wen

E-mail:nlo@thu.edu.tw

羅能文(第六家族)Lo, Neng-Wen
職稱
Title
副教授暨系主任
Associate Professor/Chair
學生訪談時間
Students time
星期二 10:00~12:00 ; 星期三 16:00~17:00
Tues. 10:00~12:00 Wed. 16:00~17:00
聯絡方式
Contact
Tel: 886-04-23590121轉37115
Fax: 886-04-23590385 
E-mail: nlo@thu.edu.tw
經歷
Background
東海大學物理學系學士
美國紐約州立大學生物化學系博士
美國約翰霍普金斯醫學院博士後研究員
B.S., Dept. of Physics, Tunghai University
Ph.D., State University of New York at Buffalo, USA
Fellow, Dept. of Tumor Biology, Johns Hopkins School of Medicine, USA
研究方向
Research

1. Characterization of gene promoter regions by bio-informatic algorithms
2. Studies of germ cell differentiation and isolation of stem cells
3. Mechanisms of glycoprotein modifications
授課名稱
Courses
生物科技概論 (0-2) 
Introduction to Biotechnology (0-2) 
基礎分子生物學 (2-0) 
Introdution to Molecular Biology (2-0) 
生殖細胞生物學 (2-2) 
Germ Cell Biology (2-2) 
生物技術實驗 (1-1) 
Biotechniques (1-1) 
生物分子檢測 (0-2) 
Assays on Biomolecules (0-2) 
生物資訊電腦搜尋(0-2)
Computer Search of Biological Information (0-2)

1. Characterization of gene promoter regions by bio-informatic algorithms

2. Studies of germ cell differentiation and isolation of stem cells

3. Mechanisms of glycoprotein modifications

  1. Lin, N.S., Lin, B.Y. Lo, N.W., Hu, C.C., Chow, T.Y. & Hsu, Y.H. 1994. Nucleotide sequence of the genomic RNA of bamboo mosaic potexvirus. Journal of General Virology 75 ( Pt 9),2513-8. (SCI)
  2. Lo, N.W. & Lau, J.T. 1996. Transcription of the β-galactoside α 2,6-sialyltransferase gene in B lymphocytes is directed by a separate and distinct promoter. Glycobiology 6(3):271-9. (SCI)
  3. Lo, N.W. & Lau, J.T. 1996. Novel heterogeneity exists in the 5′-untranslated region of the -galactoside α 2,6-sialytransferase mRNAs in the human B-lymphoblastoid cell line, Louckes. Biochemical & Biophysical Research Communications 228(2):380-5. (SCI)
  4. Shaper, N.L., Charron, M., Lo, N.W. & Shaper, J.H. 1998. β4-Galactosyltransferase and lactose biosynthesis: Recruitment of a housekeeping gene from the nonmammalian vertebrate gene pool for a mammary gland specific function. J. Mammary Gland Biology and Neoplasia 3(3):315-24.(SCI)
  5. Lo, N.W., Shaper, J.H., Pevsner, J. & Shaper, N.L. 1998. The expanding β4-galactosyltransferase gene family: messages from the databanks. Glycobiology 8(5):517-26. (SCI)
  6. Lo, N.W. & Lau, J.T. 1999. Transcription of the β-galactoside 2,6-sialyltransferase gene (SIAT1) in B lymphocytes: Cell type-specific expression correlates with presence of the divergent 5’-untranslated sequence. Glycobiology 9(9): 907-914. (SCI)
  7. Lo, N.W., Dennis, J. W. & Lau., J. T. Y. 1999. Overexpression of the alpha2,6-sialyltransferase, ST6Gal I, in a low metastatic variant of a murine lymphoblastoid cell line is associated with appearance of a unique ST6Gal I mRNA. Biochemical &Biophysical Research Communications 264 (3):619-621. (SCI)
  8. Lo, N. W., Changchien, S. W., Chang, Y. F. and Lu, T. C. 2002. Human promoter prediction based on sorted consensus sequence patterns by genetic algorithms. Proceedings of the International Congress on Biological and Medical Engineering. D3I-1540: pp 111-112.
  9. 朱俐潔、蘇郁欣、施宗雄、閻立平、羅能文。2003。生乳中產 β 1,4-半乳糖分解酵素低溫菌之篩選。東海學報 44: 59-65。
  10. Lo, N. W., Changchien, S. W., Chang, Y. F. and Lu, T. C. 2003. Improved promoter prediction by two-phase neural network. Proceedings of the International Conference on Mathematics and Engineering Techniques in Medicine and Biological Science pp 42-46{ISBN = 1-932415-04-1}
  11. Chang, H. T., Lo, N. W., Lu, W. C. and Kuo, C. J. 2003. Visualization and comparison of DNA sequences by use of three-dimensional trajectories. Proceedings of the Conferences in Research and Practice in Information Technology pp 81-85. {ISBN ~ ISSN:1445-1336 , 0-909-92597-6}
  12. Chang, H.T., Xiao, S.W., Lin, C.C. & Lo, N.W. 2004. “Feature Extraction based on Three-Dimensional Trajectory (TDT) of DNA Sequences,” Workshop on Consumer Electronics and Signal Processing (WCEsp2004), pp. 459~463, 2004/11
  13. Yeh, Y. C., Yang V.C., Huang, S. C. and Lo, N. W. 2005. Stage-dependent expression of ESX1 protein: a candidate marker for X-chromosome-bearing sperm. Reproduction, Fertility and Development 17(4): 447-455. (SCI)
  14. 蘇郁欣、羅能文。2005。耐低溫Serratia屬D04菌株β1,4-半乳糖??之定性分析與基因選殖。中國畜牧學會會誌34(3):189-198。
  15. Leu, F.Y., Lo, N.W. and Yang, L.N. 2005. Predicting Vertebrate Promoters with Homogeneous Cluster Computing, IEEE SITIS, pp. 143-148.
  16. Lo, N.W., Chang, H.T., Xiao, S.W., Li, C.H. & Kuo, C.J. 2007. Global visualization and comparison of DNA sequences by use of three-dimensional trajectories. J. of Info. Sci. and Eng. 23 :1723-1736. (SCI)
  17. Intawicha, P., Ou, Y.W., Lo, N.W., Zhang, S.C., Chen, Y.Z., Lin, T.A., Su, H.L., Guu, H.F., Chen, M.J., Lee, K.H., Chiou, W.T. and Ju, J.C. 2009. Characterization of embryonic stem cell lines derived from New Zealand White rabbit embryos. Cloning and Stem Cells 11(1):27-38. (SCI)
  18. Nguyen, N. T., Lin, P.C., Siriboon, C., Lo, N. W. and Ju, J. C. 2010. Sonic Hedgehog improves in vitro development of porcine parthenotes and handmade cloned embryos. Theriogenology 74(4):1149-1160. (SCI)
  19. 謝亞振、殷永山、羅能文、李坤雄、朱志成。2010。綜合評論:源自孤雌胚胚幹細胞之特性與應用。台灣農學會報11(6):580-601。
  20. Leu, F.Y., Yang, L.N., Lo, N.W. and Lin, I. L. 2010. Predicting vertebrate promoters using heterogeneous clusters. Int. J. of Ad Hoc and Ubiquitous Computing 6(4): 216-234. (SCI)
  21. Hsieh, Y.C., Intawicha P., Lee, K.H., Chiu, Y.T., Lo, N.W. and Ju, J.C. 2011. LIF and FGF Cooperatively Support Stemness of Rabbit Embryonic Stem Cells Derived from Parthenogenetically Activated Embryos. Cell Reprogram 13(3):241-55.(SCI)
  22. Nguyen, N.T., Lo, N.W., Chuang, S. P., Jian, Y.L. and Ju, J. C. 2011. Sonic Hedgehog supplemented oocyte and embryo culture media enhances development of in vitro fertilized porcine embryos. Reproduction 142(1):87-97.(SCI)
  23. Chawalit, S., Nguyen, N.T., Tseng, J.K., Lo, N.W., Tu, C.F., and Ju, J.C. 2012. Trichostatin A and ascorbic acid assist in the development of porcine handmade cloned embryos via different physiologic pathways. Reprod Sci. 19(9):976-86.
  24. Chang, H.T., Kuo, C. J., Lo, N.W., Lv, W.Z. 2012. DNA Sequence Representation and Comparison Based on Quaternion Number System. Int. J. Adv. Comput. Sci. Appl. 3(11): 39-46.
  25. Kere, M., Siriboon, C., Lo, N.W., Nguyen, N.T., and Ju, J.C. 2013. Ascorbic Acid Improves the Developmental Competence of Porcine Oocytes after Parthenogenetic Activation and Somatic Cell Nuclear Transplantation. J. Reprod. Dev. 59(1):78-84.
  26. Intawicha P, Wang SH, Hsieh YC, Lo NW, Lee KH, Huang SY, Ju JC. 2013. Proteomic profiling of rabbit embryonic stem cells derived from parthenotes and fertilized embryos. PLoS One. 8(7):e67772.
  27. Kere M, Siriboon C, Liao JW, Lo NW, Chiang HI, Fan YK, Kastelic JP, Ju JC. 2014. Vascular endothelial growth factor A improves quality of matured porcine oocytes and developing parthenotes. Domest Anim Endocrinol. 49C:60-69.
  28. Siriboon C, Tu CF, Kere M, Liu MS, Chang HJ, Ho LL, Tai ME, Fang WD, Lo NW, Tseng JK, Ju JC. 2014. Production of viable cloned miniature pigs by aggregation of handmade cloned embryos at the 4-cell stage. Reprod Fertil Dev. 26(3):395-406.
  29. Lo, N.W., Intawicha, P., Chiu, Y.T., Lee, K.H., Lu, H.C. , Chen, C.H., Chang,Y.H., Chen, C.D. and Ju, J.C. 2015. Leukemia Inhibitory Factor andFibroblast Growth Factor-2 Critically and Mutually Sustain Pluripotency of
    Rabbit Embryonic Stem Cells. Cell Transplantation.
  30. Intawicha, P., Siriboon, C., Chen, C.H., Chiu, Y.T., Lin, T.A., Kere, M., Lo,N.W., Lee, K.H., Chang, L.Y., Chiang, H.I.,and Ju, J.C. 2016. Derivation andcharacterization of putative embryonic stem cells from cloned rabbit embryos.Theriogenology 86(7):1799-810.
  31. Wang, D.C., Huang, J.C., Lo, N.W., Chen, L.R., Mermillod, P., Ma, W.L.,Chiang, H.I., and Ju, J.C. 2017. Sonic Hedgehog promotes in vitro oocytematuration and term development of embryos in Taiwan native goats.
    Theriogenology 103:52-58.
  32. Lumsangkul, C., Chiang, H.I., Lo, N.W., Fan, Y.K., and Ju, J.C. 2019.Developmental Toxicity of Mycotoxin Fumonisin B₁ in Animal Embryogenesis: An Overview. Toxins 13:11(2). pii: E114. doi: 10.3390/toxins11020114. Review.​(MOST 106-2313-B-039-003-MY2.)
  33. Intawicha, P., Tsai, L.K., Yen, S.Y., Lo, N.W., and Ju, J.C. 2019. Nucleus,Cytoskeleton, and Mitogen-Activated Protein Kinase p38 Dynamics during In Vitro Maturation of Porcine Oocytes. Animals 13;9(4). pii: E163. doi: 10.3390/ani9040163.(MOST 106-2313-B-039-003-MY2.)
  34. Kere, M., Liu, P.C., Chen, Y.K., Chao, P.C., Tsai, L.K., Yeh, T.Y., Siriboon, C., Intawicha, P., Lo, N.W., Chiang, H.I., Fan, Y.K., and Ju, J.C.. Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes. Animals (Basel). 2020 Apr 11;10(4):664. doi: 10.3390/ani10040664. PMID: 32290459; PMCID: PMC7222836.
  35. Chen, J.S., Tsai, L.K., Yeh, T.Y., Li, T.S., Li, C.H., Wei, Z.H., Lo, N.W., and Ju, J.C. Effects of electromagnetic waves on oocyte maturation and embryonic development in pigs. J Reprod Dev. 2021 Dec 14;67(6):392-401. doi: 10.1262/jrd.2021-074. Epub 2021 Oct 23. PMID: 34690215; PMCID: PMC8668371.
  36. Lee, C.C., Wang, T.C., Wang, H.Y., Hsu, B., Shih, R.J., Lo, N.W., and Wang, C.Y.. Association Between HSPA5 Promoter Polymorphisms and a Reduced Risk of Normal Tension Glaucoma. Ophthalmic Res. 2022 Apr 4. doi: 10.1159/000524173. Epub ahead of print. PMID:35378534.
  1. Grollman, E.F., Lo, N.W. & Lau, J.T. 1992. Regulated expression of β-galactoside 2,6 sialyltransferase in rat FRTL-5 cells. Glycobiology 2, 478
  2. 2.Lo, N.W., Vertino-Bell, A., Hu, Y.P. & Lau, J.T. 1994. 5’ heterogeneity in mRNAs expressed from the β-galactoside 2,6-sialyltransferase gene, Glycobiology 4, 739.
  3. Lo, N.W. & Lau, J.T. 1995. Transcriptional regulation of β-galactoside 2,6-sialyltransferase in B lymphocytes. Glycoconjugate J. 12, 470.
  4. Lo, N.W. & Lau, J.T. 1996. Transcriptional regulation of β-galactoside 2,6-sialyltransferase in B lymphocytes. Glycobiology 6, 721.
  5. Lo, N.W., Shaper, J.H., Pevsner, J.& Shaper, N.L. 1998. The mouse β4-galactosyltransferase gene family. FASEB 12, A1348.
  6. Lo, N.W., Shaper, J.H. & Shaper, N.L. 1999. Differential expression of the murine β1,4-galactosyltransferase gene family members in male germ cells during spermatogenesis. c 16, S73.
  7. Lo, N.W., Shaper, J.H., Pevsner, J.& Shaper, N.L. (1999). The mouse β4-galactosyltransferase gene family. J. Chin. Soc. Anim. Sci. 28 (Suppl.): 79.
  8. Lo, N.W., Shaper, J.H. & Shaper, N.L. 2000. Differential expression of the murine β1,4-galactosyltransferase gene family members in murine male germ cells. J. Chin. Soc. Anim. Sci. 29 (Suppl.): 148.
  9. Huang, S.C., Hu, C.C. & Lo, N.W. 2000. Generation of polyclonal antibodies against the mouse homeobox protein Esx1. J. Chin. Soc. Anim. Sci. 29 (Suppl.): 149.
  10. Ku, M.C. & Lo, N.W. 2000. Tissue-specific expression of the Esx1 gene correlates with its heterogeneous mRNA isoforms. J. Chin. Soc. Anim. Sci. 29 (Suppl.): 150.
  11. Chu, L.C., Shih, T.H. & Lo, N.W. 2001. Characterization of the β1,4-galactosidase activity in psychrotrophs. J. Chin. Soc. Anim. Sci. 30 (4): 113.
  12. Ku, M.C. & Lo, N.W. 2001. Effects of stress on Esx1 gene expression in embryogenesis. J. Chin. Soc. Anim. Sci. 30 (4): 194.
  13. Yeh, Y. C., Huang, S. C. and Lo, N. W. 2002. Stage-dependent expression of the esx1 in mouse testis. Proceedings of the 4th Asian-Pacific Organization for Cell Biology Congress. POS9-5: pp257.
  14. Wang, P.Y. & Lo, N.W. 2002. Cloning of the genes that differentiate primary spermatocytes from spermatogonia. J. Chin. Soc. Anim. Sci. 31(4):70.
  15. Yeh, Y.C., Huang, S. C. & Lo, N.W. 2002. The apecific expressionof the Esx1 in the testis and spermatozoa. J. Chin. Soc. Anim. Sci. 31(4):198.
  16. Lo, N.W., Changchien, S.W., Chang, Y.F. 2003. Messages from the sequence comparison between the transcription factor binding sites and extracted promoter consensus sequences. Proceedings of the2003 Bioinformatics in Taiwan, Symposium and Workshop. pp110.
  17. Lo, N. W., Chang, Y. F. and Changchien, S. W. 2004. Sequence comparison between the transcription factor binding sites and extracted promoter consensus sequences. Proceedings of the Yeast Genetics and Molecular Biology Meeting 2004. pp.227.
  18. Chang, H.T., Xiao, S.W. & Lo, N.W. 2005 Feature extraction and comparison of TDTs: an efficient sequence retrieval algorithm for genomic databases. Proceedings of the Third Asia-Pacific Bioinformatics Conference & Singapore Bioinformatics Week. pp75.
  19. Yeh, Y.C., Yang, V.C. and Lo, N.W. 2005. In vitro analyses of the putative promoter region of Esx1 gene in testis and placenta. Proceedings of the 38th Annual Meeting of the Society for the Study of Reproduction 164, pp118-119.
  20. Lo, N.W. , Cheng, R.S., Yeh, Y.C. and Yang, V.C. 2007 Characterization of the regulatory elements of the spermatogenesis-specific Esx1 gene. BIOLOGY OF REPRODUCTION 77, 117-a–117.
  21. Wang, M.C., Lo, N.W.& Chang, H.T. 2008. Monitored and inspected mouse mating by video surveillance. Journal of The Chinese Society of Animal Science , vol. 37(Supplement), pp. 113, Taipei, 2008/12/7.
  22. Hsieh, Y.C., Intawicha, P., Lo, N.W., Lee, K.H. and Ju, J.C. 2009. Establishment and characterization of rabbit embryonic stem cell lines derived from parthenogenetically activated embryos. “The International Symposium of Stem Cells and Bioengineering” on the 16th and 17th of May of 2009 at the Convention Center of Medical College of National Cheng Kung University. P2-17.
  23. Lo, N. W., Chang, H.T. & Wang, M.C. 2009. Automatic Detection of Mouse Mating Behavior by Video Surveillance,” APSIPA Annual Summit and Conference (APSIPA ASC 2009), Sapporo, Japan, October 4-7, TP-P2-5: 0149.
  24. 24.Hsieh,Y.C., Intawicha, P., Lo, N.W. and Ju, J. C. 2009. Establishment and characterization of rabbit embryonic stem cell lines derived from parthenogenetically activated embryos.Annual meeting of Taiwan Society for Stem Cell Research, TSSCR 2009.(Suppl.):116.
  25. 25.Intawicha, P., Hsieh, Y.C., Lo, N.W., Lee, K.H. and Ju, J. C. 2010. Synergistic integration of LIF and FGF pathway supports self-renewal of Rabbit embryonic stem cells. 3rd Conference of the Asia Pacific Initiative on Reproduction, ASPIRE 2010.
  26. 26.Ju, J.C., Intawicha, P., Wang, S.H., Hsieh, Y.J., Lo, N.W. and Huang, S.Y. 2010. Proteomic Analysis of Rabbit Embryonic Stem Cells Derived from Fertilized and Parthenogenetically Activated Embryos. Biology of Reproduction (Suppl):199-200.
  27. Lo, N.W., Intawicha P., Hsieh, Y. C., Lee, K.H., Chen, L.R. and Ju, J.C. 2010. Cloning rabbit embryonic stem cells. 14th Animal Science Congress of the Asian-Australasian Association of Animal Production Societies (14th AAAP Animal Science Congress). (Suppl. 1) : 249.
  28. Intawicha, P., Pan, Y.H.,Chen, C.D.,Hsieh, Y.C. Tseng, J.K., Lo, N. W.,Lee, K. H. and Ju, J. C. 2010. A pilot study of intracellular calcium profiling and cell cycle synchronization in rabbit embryonic stem cells. The 8th Annual Symposium of Bioinformatics and Systems Biology in Taiwan, BIT 2010. p.75.
  29. N.W. Lo* (羅能文), C.A. Cheng, C.Y. Huang (黃啟裕), S.Y. Wang, and Y.H. Chen (陳盈豪).108.Effect of methanotropic bacteria on methane production by Taiwan male black-feathered native chickens The 2nd International Conference on Native Chicken.(ICONC 2019)泰國
  30. Chen YH, Chueh HC, Haung CY (黃啟裕)*, Lo NW (羅能文).107.Denitrification of the aerobic methanotroph Methylomonas strain THUA isolated from rumen.11th Asian Symposium on Microbial Ecology. (ASME-2019)101-101台灣
  31. J.C. Ju*, C.R. Yang, P. Intawicha, N.W. Lo (羅能文) and H.I.Chiang.104.Embryoid bodies as a model system to study effects of heat stress on post-implantation development of mammalian embryos.1st International Conference on Tropical Animal Science and Production.(TASP 2016)90泰國
  32. 闕浩丞、韓昕彤、陳怡璇、黃啓裕、江信毅、魏天恩、羅能文*。106。瘤胃嗜甲烷菌體外培養條件與 碳源降解能力最佳化研究。中國畜牧年會。46。115-台灣
  1. Shaper, N.L., Charron, M., Lo, N.W., & Shaper, J.H. (1998). β4-Galactosyltransferase and lactose biosynthesis: Recruitment of a housekeeping gene from the nonmammalian vertebrate gene pool for a mammary gland specific function. J. Mammary Gland Biology and Neoplasia, 3(3):315-24.(SCI) (review)
  2. Shaper, N.L., Charron, M., Lo, N.-W., Scocca, J.R., & Shaper, J.H. (2000). The Galactosyltransferases. In “Carbohydrates in Chemistry and Biology: A Comprehensive Handbook”, Part II (vol. III), Ernst, B., Hart, G., and Sinay, P., eds. Wiley/VCH press, New York. Chapter 10, 175-196. (book chapter)
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